HLA Class I Disease Association
HLA typing, HLA-A, B, C typing, tissue typing, HLA Class I Typing for disease association , EPIC: LAB6423, SOFT: CIDA
Specimen Collection Criteria
Collect: One Yellow-top ACD tube OR one Lavender-top EDTA tube.
Invert several times to mix well.
Physician Office/Drawsite Specimen Preparation
Do not refrigerate or freeze specimen. Maintain whole blood at room temperature (20-26°C or 68-78.8°F) prior to transport. Transport to the Laboratory within 24 hours of collection.
Preparation for Courier Transport
Transport: Whole blood at room temperature (20-25°C or 68-77°F).
- Unlabeled specimens.
- Specimens drawn in clot, SST, or Sodium Heparin tubes.
Results typically available within 5 business days.
Polymerase Chain Reaction (PCR).
HLA Class I genotyping defines the antigens of the HLA-A, B and C loci of the Major Histocompatibility Complex found on the short arm of chromosome 6. Using DNA extracted from leukocytes, HLA-A, B and C alleles are amplified via PCR using sequence-specific primers. After amplification, the specific HLA antigens are determined by gel electrophoresis. HLA genotyping serves an important role in transplantation and disease association studies.
EPIC: LAB6423, SOFT: CIDA