HLA Class I Disease Association

HLA typing, HLA-A, B, C typing, tissue typing, HLA Class I Typing for disease association , EPIC: LAB6423, SOFT: CIDA

Specimen Collection Criteria

Collect: One Yellow-top ACD tube OR one Lavender-top EDTA tube.

Invert several times to mix well.

Physician Office/Drawsite Specimen Preparation

Do not refrigerate or freeze specimen. Maintain whole blood at room temperature (20-26°C or 68-78.8°F) prior to transport. Transport to the Laboratory within 24 hours of collection.

Preparation for Courier Transport

Transport: Whole blood at room temperature (20-25°C or 68-77°F).

Rejection Criteria

  • Unlabeled specimens.
  • Specimens drawn in clot, SST, or Sodium Heparin tubes.


As needed.
Results typically available within 5 business days.

Test Methodology

Polymerase Chain Reaction (PCR).


By report.

Clinical Utility

HLA Class I genotyping defines the antigens of the HLA-A, B and C loci of the Major Histocompatibility Complex found on the short arm of chromosome 6. Using DNA extracted from leukocytes, HLA-A, B and C alleles are amplified via PCR using sequence-specific primers. After amplification, the specific HLA antigens are determined by gel electrophoresis. HLA genotyping serves an important role in transplantation and disease association studies.

CPT Code

81373, 81372.

Test Codes


Last Updated