White Faced Hornet (Allergen Specific IgE)
White Faced Hornet Venom (i2) , Antrim #30030, EPIC: LAB5738, SOFT: EWHF
Specimen Collection Criteria
Collect: One Gold-top SST tube.
Twenty individual allergen assays or allergen screens can be performed on one 5 mL Gold-top SST tube. Each allergen assay requires 100 mcL of serum.
Physician Office/Drawsite Specimen Preparation
Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Refrigerate (2-8°C or 36-46°F) the centrifuged collection tube within two hours of collection.
Preparation for Courier Transport
Transport: Centrifuged collection tube, refrigerated (2-8°C or 36-46°F).
- Plasma specimens.
- Severely lipemic or hemolyzed specimens.
0,Centrifuged SST tubes may be transported
and stored at ambient temperatures (2o to 30o C; 36o to 86o
F) for up to 48 hours. DO NOT FREEZE TUBES CONTAINING BLOOD. For longer
storage, the serum should be removed from the gel and refrigerated (2o
- 8o C) or frozen at -20o C or below.
Red-top tubes and Microtainers® may be stored at ambient temperatures
for up to 16 hours and for up to 3 days at refrigerator temperatures (2o
- 8o C). DO NOT FREEZE TUBES CONTAINING BLOOD. For longer storage,
the serum should be removed from the clot and refrigerated (2o - 8o
C) or frozen at - 20o C or below.
0,Serum specimens (pour-overs) may be stored
at room temperature (20o to 26o C; 68o to 79o F) for up
to 1 week, refrigerator temperature (2o to 8o C; 36o to 47o
F) for two weeks, and at -20o C (-4o F) for up to 3 months. Specimens
stored at - 70o C (-94o F) can be stored indefinitely.
Monday - Friday.
Results available the next business day.
Allergy Reference Range: Less than 0.35 kU/L.
|Less than or equal to 0.34
|Greater than 100
Fluorescence Enzyme Immunoassay (FEIA).
The allergen class may not be predictive of clinical disease in some patients. The diagnosis of allergy should be based upon patient history and clinical findings. The diagnosis of allergy should not be based upon laboratory findings alone.
Positive assay results indicate a high probability of allergic disease. Negative assay results effectively rule out allergy induced by those allergens.
In the United States it is estimated that one to two million people are severely allergic to stinging insect venom. Approximately 90 to 100 deaths occur annually from sting reactions. This number may under-represent the true total because some sting reactions are misdiagnosed as heart attacks, sunstroke, or other acute illnesses. More people die each year from the effects of insect venom than from spider or snake bites. One person in 100 who is stung by an insect can develop a fatal reaction.
Typically, an insect sting produces local redness and swelling that resolves in a few hours. Reactions in an allergic individual develop within a few minutes and are more severe than in normal patients. Symptoms of an allergic reaction include intense redness at the sting site, swelling spanning two joints, itching, and pain. A major allergic reaction includes focal swelling, itching, faintness, sweating, headache, stomach cramps, vomiting, diarrhea, constrictive chest, difficulty breathing and swelling of the throat. Severe cases can lead to anaphylactic shock and death. Death can occur without a previous history of a sting allergy. The frequency of fatal reactions due to anaphylaxis also increases with age (1).
The primary allergens of vespid (Yellow Jacket, Hornet, and Wasp) venoms include antigen 5 (nonenzymatic protein), phospholipase A, and hyaluronidase. There are physiological and immunological differences between the honey bee and vespid venoms. However, IgE tests for these allergens do not cross-react (1).
Prophylactic measures must be taken for those individuals who develop severe reactions to insect stings. Specific immunotherapy (hyposensitization) remains the most effective means of treatment. Hyposensitization has a 96% success rate against insect stings.
- Kaplan, Allen P. M.D. Allergy. Churchill Livingstone: New York, pg 508-509, 1985.
Antrim #30030, EPIC: LAB5738, SOFT: EWHF
Microtainer® and Vacutainer® are registered trademarks of Becton, Dickinson and Company.
UroVysion® is a registered trademark of Abbott Laboratories. ThinPrep® is a registered trademark of Hologic, Incorporated.
This directory currently reflects information only for specimens collected and/or processed at the
Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.