Lab Test

Influenza A H1N1 (2009) By PCR

novel influenza, nH1N1(2009), nH1N1, H1N1, swine influenza, swine flu, Influenza A nH1N1 (2009) by RT-PCR, SOFT: IFLUG

Test Codes


Specimen Collection Criteria

NP swab in viral transport medium (i.e. M4-RT, M5, Universal Transport Media) refrigerated (2-8°C or 36-46°F).
During collection, please remember to leave the NP swab in the patient's nasopharynx for 30 seconds and to gently rotate the swab to obtain an adequate specimen.

* Testing nasopharyngeal aspirates/washes and bronchoalveolar lavage/wash specimens must be approved by the Medical Director; otherwise these samples should be tested by viral culture (refer to comprehensive viral culture).

Physician Office/Draw Specimen Preparation

Store refrigerated or on wet ice (2-8°C or 36-46°F).

Preparation for Courier Transport

Transport specimens refrigerated or on wet ice (2-8°C or 36-46°F).

Rejection Criteria

Samples collected in bacterial transport system, dry swabs, plasma or serum cannot be tested.

Inpatient Specimen Preparation

Transport specimens to the Royal Oak Clinical Molecular Pathology laboratory refrigerated/wet ice (2-8°C or 36-46°F) as soon as possible.


Room temperature (20-25°C or 68-77°F): 2 hours
Refrigerated (2-8°C or 36-46°F): 3 days


Royal Oak Clinical Molecular Pathology Laboratory


Sunday - Saturday, batched
Results available usually within 24 hours.

Reference Range

Negative (No influenza A viruses detected)

Test Methodology

Real-time, Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR)


By report.

Clinical Utility

Detects all types of influenza A viruses and specifically detects the H1N1 (2009) influenza A virus, formerly "Swine Flu".

This test may be used to differentiate seasonal influenza A from the nH1N1 (2009) influenza A virus. For additional information please contact the Customer Service via 1-800-551-0488, option 5.

CPT Codes



Last Updated


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This directory currently reflects information only for specimens collected and/or processed at the
Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.