Lab Test

Histone Antibody (IgG)

Anti-Histone Antibody , Antrim #30375, EPIC: LAB5761, SOFT: HISG

Specimen Collection Criteria

Collect: One Gold-top SST tube. (Minimum Whole Blood: 2.0 mL)

Physician Office/Drawsite Specimen Preparation

Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Refrigerate (2-8°C or 36-46°F) the centrifuged collection tube within two hours of collection. (Minimum Serum: 0.5 mL)

Preparation for Courier Transport

Transport: Centrifuged collection tube, refrigerated (2-8 °C or 36-46 °F). (Minimum Serum: 0.5 mL)

Rejection Criteria

  • Plasma specimens.
  • Severely hemolyzed, lipemic or icteric specimens. 

Storage

0,Centrifuged SST tubes may be transported and stored at ambient temperatures (2o to 30oC; 36 to 86oF) for up to 48 hours. DO NOT FREEZE TUBES CONTAINING BLOOD. For longer storage, the serum should be removed from the gel and refrigerated (2o-8oC) or frozen at -20oC or below.

Red-top tubes and Microtainers® may be stored at ambient temperatures for up to 16 hours and for up to 3 days at refrigerator temperatures (2o-8oC). DO NOT FREEZE TUBES CONTAINING BLOOD. For longer storage, the serum should be removed from the clot and refrigerated (2o-8oC) or frozen at -20oC or below.

0,Serum specimens (pour-overs) may be stored at room temperature (20o to 26oC; 68 to 79oF) for up to 1 week, refrigerator temperature (2 to 8oC; 36 to 47oF) for two weeks, and at -20oC (-4oF) for up to 3 months. Specimens stored at -70oC (- 94oF) can be stored indefinitely.

Performed

Monday – Friday.
Results available within 2 business days.

Reference Range

Negative: Less than 100 AU/mL.
Equivocal: 100-120 AU/mL.
Positive: Greater than 120 AU/mL.

Test Methodology

Multiplex Bead Immunoassay.

Interpretation

Histone antibodies are present in 20-55% of idiopathic SLE and 80-95% in drug induced SLE. They occur in less than 20% of other types of connective tissue diseases.

Clinical Utility

The histone antibody assay aids in the diagnosis of SLE.

CPT Code

86235

Test Codes

Antrim #30375, EPIC: LAB5761, SOFT: HISG

Last Updated

7/11/2019

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This directory currently reflects information only for specimens collected and/or processed at the
Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.