Lab Test

Enterovirus by Nucleic Acid Amplification

Meningitis, Viral, Virus, URI, Upper Respiratory Infection, PCR, Real-Time PCR , WARDE: EVPCR, EPIC: LAB6860, SOFT: IEVNA

Specimen Collection Criteria

Collect: One of the following specimen types:

  1. Cerebrospinal Fluid (CSF) in a sterile collection container. (Min: 0.5 mL)
    • Specimen must be refrigerated within one hour or collection.
  2. Nasopharyngeal (NP) Swab in viral transport media (UVT, UTM).
  3. Bronchoalveolar lavage/wash in sterile screw-capped plastic container 1.0 mL (Min: 0.5 mL).
  4. Stool in sterile screw-capped plastic container 2.0 mL (Min: 1.0 mL) for liquid stool or marble-size solid stool.
  5. Urine in a sterile screw cap container (Min: 1.0 mL)
  6. Bone Marrow in EDTA, 1.0 mL (Min: 0.5 mL).
  7. Whole blood in EDTA, 2.0 mL (Min: 1.0 mL).
  8. Swab specimens (throat, oral, dermal, ocular, esophagus) in viral transport medium (UVT, UTM).
  9. Biopsy/tissue specimens in saline or viral transport medium (Snap frozen -20°C).

Physician Office/Drawsite Specimen Preparation

  • CSF: Maintain specimen refrigerated (2-8°C or 36-46°F). Specimens not received in the Laboratory within 24 hours of collection must be frozen (-20°C/-4°F or below).
  • NP Swabs: Maintain specimen in viral transport medium (UVT, UTM), refrigerated (2-8°C or 36-46°F) or frozen (-20°C/-4°F or below).
  • Biopsy/tissue specimens:  Maintain specimen frozen (-20°C/-4°F or below)

For all other specimen types: Maintain specimen refrigerated (2-8°C or 36-46°F).

Preparation for Courier Transport

Transport: CSF in a sterile collection container (Min: 0.5 mL), NP swab in viral transport medium, throat, oral, dermal, ocular, or esophagus swabs in viral transport medium, bone marrow in EDTA (Min: 0.5 mL), whole blood in EDTA (Min: 1.0 mL), biopsy/tissue specimens in saline or viral transport medium: refrigerated (2-8°C or 36-46°F) or frozen (-20°C/-4°F or below).

Rejection Criteria

  • Specimens submitted on dry swabs.
  • Specimens submitted in bacterial transport systems.
  • Specimens submitted on cotton or calcium alginate swabs, or on wooden shaft swabs.


CSF and NP Swabs: Sunday - Saturday.
Results available within 24-48 hours.

All other sample types: Monday – Saturday

Results available 2-4 days.

Reference Range


Test Methodology

Nucleic Acid Amplification (FDA Approved).


A negative result does not rule out Enterovirus infection.

Clinical Utility

This assay provides a highly sensitive and specific test for the diagnosis of enteroviral infection.

Clinical Disease

The Enteroviruses consist of a group of small RNA viruses within the family Picornaviridae and include the poliovirus and the non-polioviruses: coxsackieviruses group A and B, echoviruses and the numbered enteroviruses. Enterovirus infections include aseptic meningitis, encephalitis, and upper respiratory tract infections.

CPT Code


Test Codes


Last Updated


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This directory currently reflects information only for specimens collected and/or processed at the
Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.