Creutzfeldt Jacob Disease (CJD) Immunoassay
CJD proteins: 14-3-3 beta, RT-QuIC, Total tau
Test Codes
EPIC: LAB6519, Beaker: XCJD, National Prion
Department
Send Outs
Specimen Collection Criteria
Collect: 5.0 mL Cerebrospinal Fluid (CSF) in a clean plastic screw-cap tube. Avoid a bloody tap. (Minimum: 2.0 mL). Freeze sample immediately after collection, preferably within 30 minutes.
- Collect CSF using standard lumbar puncture procedure.
- Discard the first 2.0 mL of fluid or use it for other testing.
Physician Office/Draw Specimen Preparation
Freeze (-20°C/-4°F or below) immediately and maintain frozen prior to transport.
Preparation for Courier Transport
Transport: 5.0 mL CSF frozen (-20°C/-4°F or below). (Minimum: 2.0 mL)
Rejection Criteria
Specimens not collected and processed as indicated.
In-Lab Processing
Freeze (-20°C/-4°F or below) immediately prior to transport.
Transport: 5.0 mL CSF frozen (-20°C/-4°F or below). (Minimum: 2.0 mL)
Storage
Specimen Stability for Testing:
Room Temperature (20-26°C or 68-78.8°F): unacceptable
Refrigerated (2-8°C or 36-46°F): unacceptable
Frozen (-20°C/-4°F or below): Indefinite
Specimen Storage in Department Prior to Disposal:
Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.
Laboratory
Sent directly to National Prion Laboratory, Cleveland, OH.
Performed
Tuesday and Thursday.
Results available within 8-14 days.
Reference Range
By report.
Test Methodology
Immunoassay and Real-Time Quaking-Inducing Conversion (RT-QuIC).
Interpretation
By report.
Clinical Utility
In patients with dementia, a positive immunoassay for the 14-3-3 brain protein in cerebrospinal fluid strongly supports a diagnosis of Creutzfeldt-Jakob disease. This finding, however, does not support the use of the test in patients without clinically evident dementia.
CPT Codes
86317, 84182, 0035U.
Contacts
Send Outs Laboratory – RO
248-551-9045
Name: Send Outs Laboratory – RO
Location:
Phone: 248-551-9045
Last Updated
10/27/2023
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