Aggressive B-cell Lymphoma Panel by Fluorescence In Situ Hybridization (FISH) Analysis
High-Grade B-Cell Lymphoma, with MYC and BCL2 , and/or BCL6 Rearrangements (Aggressive B-cell Lymphoma, IgK (lg Kappa chain) gene rearrangement for Burkitt’s lymphoma, IgL (lg Lambda chain) gene rearrangement for Burkitt’s lymphoma, MYC gene rearrangement for Burkitt’s lymphoma-associated translocations, IgH/MYC fusion for t(8;14) detection, Please contact the Cytogenetics Laboratory if assistance with ordering in EPIC or SOFT is needed.
Please contact the Cytogenetics Laboratory if assistance with ordering in EPIC or SOFT is needed.
Specimen Collection Criteria
Collect: Bone marrow aspirate in a Dark Green-top Sodium Heparin tube, lymph node touch preparation, or paraffin-embedded tissue section.
A copy of the requisition must be sent with the specimen.
Physician Office/Draw Specimen Preparation
Do not freeze specimen. Maintain specimens at room temperature (20-25°C or 68-77°F) prior to courier pickup. For delays in transport (greater than 24 from the time of collection), refrigerate (2-8°C or 36-46°F) the specimen.
Preparation for Courier Transport
Transport: Bone marrow, lymph node touch preparation, or paraffin-embedded tissue, at room temperature (20-26°C or 68-78.8°F) or refrigerated (2-8°C or 36-46°F).
- Specimens arriving in the Laboratory 4 days or more following the original collection date.
- Frozen or fixed specimens.
Specimens are processed immediately upon receipt in the Laboratory.
Specimen Stability for Testing:
Room Temperature (20-26°C or 68-78.8°F): 24 hours
Refrigerated (2-8°C or 36-46°F): 96 hours
Frozen (-20°C/-4°F or below): Unacceptable
Specimen Storage in Department Prior to Disposal:
Refrigerated (2-8°C or 36-46°F): 7 days. A backup cell pellet is maintained for three weeks after the case has been signed out.
Royal Oak Clinical Cytogenomics Laboratory
Monday – Friday, 8:00 am – 5:00 pm.
Results available within 7 days of receipt in the Laboratory.
Positive or negative for a neoplastic clone. An interpretative report will be provided.
Fluorescence In Situ Hybridization (FISH) Analysis.
A positive FISH result indicates the presence of MYC, IGH/BCL2, and/or BCL6 gene rearrangement.
The presence in a B-cell lymphoma of two or three of the abnormalities identified in this FISH panel predict a poor prognosis that requires aggressive treatment. This assay can be performed on a bone marrow biopsy, lymph node touch preparation, or a paraffin-embedded tissue section.
For MYC Gene Rearrangement: 88271x1, DNA probe, each.
For IGH/MYC: 88271x3.
For Ig Kappa or Ig Lambda: 88271x1, DNA probe, each.
For All Assays: 88275x1, Interphase analysis, 100-300 cells.
Cytogenetics Laboratory – RO
Microtainer® and Vacutainer® are registered trademarks of Becton, Dickinson and Company.
UroVysion® is a registered trademark of Abbott Laboratories. ThinPrep® is a registered trademark of Hologic, Incorporated.
This directory currently reflects information only for specimens collected and/or processed at the
Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.