Lab Test

Aggressive B-cell Lymphoma Panel by Fluorescence In Situ Hybridization (FISH) Analysis

High-Grade B-Cell Lymphoma, with MYC and BCL2 , and/or BCL6 Rearrangements (Aggressive B-cell Lymphoma, IgK (lg Kappa chain) gene rearrangement for Burkitt’s lymphoma, IgL (lg Lambda chain) gene rearrangement for Burkitt’s lymphoma, MYC gene rearrangement for Burkitt’s lymphoma-associated translocations, IgH/MYC fusion for t(8;14) detection , Please contact the Cytogenetics Laboratory if assistance with ordering in EPIC or SOFT is needed.

Specimen Collection Criteria

Collect: Bone marrow aspirate in a Dark Green-top Sodium Heparin tube, lymph node touch preparation, or paraffin-embedded tissue section.

A copy of the requisition must be sent with the specimen. 

Physician Office/Drawsite Specimen Preparation

Do not freeze specimen. Maintain specimens at room temperature (20-25°C or 68-77°F) prior to courier pickup. For delays in transport (greater than 24 from the time of collection), refrigerate (2-8°C or 36-46°F) the specimen.

Preparation for Courier Transport

Transport: Bone marrow, lymph node touch preparation, or paraffin-embedded tissue, at room temperature (20-26°C or 68-78.8°F) or refrigerated (2-8°C or 36-46°F).

Rejection Criteria

  • Specimens arriving in the Laboratory 4 days or more following the original collection date.
  • Frozen or fixed specimens.

Performed

Monday - Friday, 8:00 a.m. - 5:00 p.m.
Results available within 7 days of receipt in the Laboratory.

Reference Range

Positive or negative for a neoplastic clone. An interpretative report will be provided.

Test Methodology

Fluorescence In Situ Hybridization (FISH) Analysis.

Interpretation

A positive FISH result indicates the presence of MYC, IGH/BCL2, and/or BCL6 gene rearrangement.

Clinical Utility

The presence in a B-cell lymphoma of two or three of the abnormalities identified in this FISH panel predict a poor prognosis that requires aggressive treatment. This assay can be performed on a bone marrow biopsy, lymph node touch preparation, or a paraffin-embedded tissue section.

CPT Code

For MYC Gene Rearrangement: 88271x1, DNA probe, each.
For IGH/MYC: 88271x3.
For Ig Kappa or Ig Lambda: 88271x1, DNA probe, each.
For All Assays: 88275x1, Interphase analysis, 100-300 cells.

Test Codes

Please contact the Cytogenetics Laboratory if assistance with ordering in EPIC or SOFT is needed.

Last Updated

7/11/2019

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This directory currently reflects information only for specimens collected and/or processed at the
Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.