Immunopathology is primarily involved in the staining of formalin fixed, paraffin embedded sections of patient tissues with a variety of antibodies. Antibodies are small protein molecules that bind to specific sites in tissues and identify the location of the specific targeted marker within the patient tissue or tumor. A panel of over 100 different antibodies is available to assist the pathologist in making a diagnosis. Often tumors or disease processes cannot be distinguished from one another based on morphology alone. This necessitates the availability of antibody panels specific for differentiating tumor types. Immunohistochemical testing provides a fast and sensitive platform for ancillary testing of patient samples. The laboratory also performs fluorescent antibody staining of fresh frozen skin and kidney biopsies. This type of testing in skin biopsies assists the pathologist in making a diagnosis in skin diseases. Testing of kidney biopsies helps the pathologist make a diagnosis in cases of renal disease and during graft surveillance after organ transplantations.
Immunopathology is vitally important to the Surgical Pathology services offered by Beaumont Laboratory. Patient care and treatment is directly affected by the ability to render a correct diagnosis in a timely manner. This testing is performed daily at the request of the pathologist assigned to each patient case.Commonly Ordered Tests
- A panel of over 100 different antibodies is available to assist the Pathologist in rendering a diagnosis in a timely manner.
- Fluorescent antibody staining of fresh frozen skin biopsies.
- Fluorescent antibody staining of fresh kidney biopsies.
The Molecular Pathology Laboratory provides a variety of molecular based assays to aid the pathologist in rendering a diagnosis on a patient sample. DNA is extracted from the patient tissue with subsequent testing performed as requested. Hormone receptor assays are performed on formalin fixed, paraffin embedded tissues from patients. Tests that are offered by the laboratory include hormone receptor analysis of all breast carcinoma cases, (estrogen, progesterone, and HER2 receptors), HER2 gene amplification assays by fluorescence in-situ hybridization (FISH) on selected breast carcinoma cases, UroVysion FISH assay to help diagnose recurrent bladder carcinoma in urine specimens, Microsatellite Instability Assays (MSI) to screen patients with colorectal carcinoma for possible inherited genetic defects, DNA quantification in hydatidaform moles, and sub typing of genital and cervical lesions for HPV to identify patients at high risk for developing cervical cancer.
The Molecular Pathology Laboratory is vitally important to the Surgical Pathology services offered by Beaumont Laboratory. Patient care and treatment is directly affected by the ability of the pathologist to render a correct diagnosis in a timely manner. Most patient's material is obtained from the Histology department which allows for timely testing of the material. Physician groups specializing in urology benefit from the timely results obtained with UroVysion FISH testing for bladder cancer. All patients with a diagnosis of breast carcinoma receive treatment and prognosis information based on these tests performed by the Molecular Pathology Laboratory.
Commonly Ordered Tests
- Hormone receptor analysis of all breast carcinoma cases (estrogen, progesterone, HER2/neu receptors.
- HER2neu gene amplification assays by fluorescence in-situ hybridization (FISH) assays.
- UroVysion assay to help diagnose recurrent bladder carcinoma.
- Microsatellite instability by PCR.