HTLV I/II Antibodies with Reflex to Confirmation
HTLV I/II Western Blot, ARUP# 51164, EPIC: LAB6059, SOFT: XHTLV
Patients with positive results by ELISA will reflex to confirmation by Western Blot.
Specimen Collection Criteria
Collect (preferred specimen): One Gold-top SST tube.
Also acceptable: One plain Red-top tube, Lavender-top EDTA tube, Green-top Sodium or Lithium Heparin tube, or Light Blue-top Sodium Citrate tube.
Send specimen for processing immediately after collection.
Physician Office/Drawsite Specimen Preparation
Centrifuge to separate serum or plasma from cells within two hours of collection. Transfer serum or plasma to a plastic transport tube and refrigerate (2-8°C or 36-46°F) immediately.
Preparation for Courier Transport
Transport: 1.0 mL serum or plasma, refrigerated (2-8°C or 36-46°F). (Min: 0.5 mL)
- Specimens not collected and processed as indicated.
Specimen Stability for Testing:
Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): Indefinitely
Specimen Storage in Department Prior to Disposal:
Specimen retention time is determined by the policy of the reference laboratory. Contact the Sendout Laboratory with any questions.
Sent to ARUP Laboratories, Salt Lake City, UT.
Monday, Wednesday, Friday.
Results available in 2-6 days.
Qualitative Enzyme-Linked Immunosorbent Assay/Qualitative Western Blot.
ARUP intends use of this assay for clinical diagnosis. This assay should not be considered for blood donor screening or associated re-entry protocols.
Human T-Cell Lymphotropic Virus-1 (HTLV-1) has been associated with adult T-cell leukemia (ATL) and may play an indirect role in the pathocenesis of some B-cell chronic lymphocytic leukemia (CLL) cases. HTLV-1 has also been associated with chronic neurologic diseases including tropical spastic paraparesis (TSP) and HTLV-associated myelopathy (HAM). (1)
This is a reportable infection and positive results will be reported to the Oakland County Health Department. For more information on reportable diseases, contact the Epidemiology Department at (248) 551-4040.
HTLV-1 is primarily endemic in southwestern Japan and the Caribbean, however, parts of sub-Saharan Africa and Central and South America also have significant seroprevalence. Increased seropositivity has been observed in the Southwestern United States, the United Kingdom, and in countries with large Caribbean populations. Approximately 0.1% of males and 0.2% of females infected at childhood develop ATL as adults. HTLV-1 appears to have very little genetic variability. (1)
10-30 years. Infection is assumed to be lifelong. HTLV-1 associated myelopathy sometimes develops just a few years after infection. (1)
HTLV-1 is poorly contagious and transmission can occur via blood transfusion, contaminated needles, sexual contact, and from mother to child through breast feeding. (1)
- Wiedbrauk D, Johnston SLG. Manual of Clinical Virology, Raven Press, New York, NY, 1993.
86790, (86689 if indicated for confirmation).
ARUP# 51164, EPIC: LAB6059, SOFT: XHTLV