HLA Class II Typing
DNA-based histocompatibility testing, disease association, histocompatibility, HLA DR testing, Donor HLA typing, Class II Typing, EPIC: LAB6422, SOFT: CIITY
Specimen Collection Criteria
Collect: Two Lavender-top EDTA tubes OR two Yellow-top ACD tubes.
Invert several times to mix well.
Physician Office/Drawsite Specimen Preparation
Do not refrigerate or freeze specimen. Maintain whole blood at room temperature (20-26°C or 68-78.8°F) prior to transport. Transport to the Laboratory within 24 hours of collection.
Preparation for Courier Transport
Transport: Whole blood at room temperature (20-25°C or 68-77°F).
- Unlabeled specimens.
- Specimens drawn in clot, SST, or Sodium Heparin tubes.
Specimen Stability for Testing:
Room Temperature (20-26°C or 68-78.8°F): 72 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable
Specimen Storage in Department Prior to Disposal:
Frozen (-80°C): 5 years (DNA)
Royal Oak HLA Laboratory
Results typically available within 5 business days.
Polymerase Chain Reaction (PCR).
HLA Class II genotyping defines the antigens of the HLA-DRB1 and DQB1 loci of the Major Histocompatibility Complex found on the short arm of chromosome 6. Using DNA extracted from leukocytes, HLA-DRB1 and DQB1 alleles are amplified via PCR using sequence-specific primers. After amplification, the specific HLA antigens are determined by gel electrophoresis. HLA genotyping serves an important role in transplantation and disease association studies.
EPIC: LAB6422, SOFT: CIITY
Microtainer® and Vacutainer® are registered trademarks of Becton, Dickinson and Company.
UroVysion® is a registered trademark of Abbott Laboratories. ThinPrep® is a registered trademark of Hologic, Incorporated.
This directory currently reflects information only for specimens collected and/or processed at the Farmington Hills,
Grosse Pointe, Royal Oak, and Troy campuses.