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Beaumont Laboratory

Lymphocyte Subset Quantitation

CD4/CD8 test, T-helper subsets, Helper/suppressor ratio, Immunodeficiency profile, Lymphocyte Subset, Flow Cytometry, Lymphocyte Subset, EPIC: LAB6416, SOFT: LYSBG

Instructions

  • Specimens should only be drawn Monday - Friday. The specimen should not be drawn on weekends or holidays. On the day before a holiday, specimens must be received in the Flow Cytometry Laboratory BEFORE 12:00 p.m. on the day before the holiday for testing to be performed.
  • A Complete Blood Count (CBC) and Differential (CBCD) is required to perform Lymphocyte Subset testing.

Specimen Collection Criteria

Collect: One Lavender-top EDTA tube for Lymphocyte Subset Quantitation AND one Lavender-top EDTA tube for Complete Blood Count with Differential. (Minimum: 2.0 mL each tube.)

  • The second Lavender-top EDTA tube at room temperature is not required for Lymphocyte Subset Quantitation if:
    • CBCD is ordered for purpose not related to this test and drawn at the same time.
    • CBCD is performed in the Outreach Client's office and results are faxed to the Flow Cytometry Laboratory (248-551-1262) and received at the time of testing.
  • If a separate specimen is NOT submitted for the Complete Blood Count with Differential, a CBCD will be ordered.
  • A copy of the requisition must be sent with the specimen. 

FedEx Shipping Instructions

Transport two 4 mL whole blood tubes (minimum: 2 mL each tube) at room temperature. Specimens must be received within 24 hours of collection. No weekend or holiday draws.

Read our complete shipping instructions.

Physician Office/Drawsite Specimen Preparation

Maintain whole blood at room temperature (20-26°C or 68-78.8°F) prior to transport. Specimens must be received in the Laboratory within 24 hours of collection.

Preparation for Courier Transport

Transport: Whole blood, at room temperature (20-25°C or 68-77°F).

Rejection Criteria

  • Clotted specimens.
  • Refrigerated specimens, accepted with approval of the Flow Cytometry supervisor or lab director.
  • Specimens received greater than 24 hours post collection. 

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 24 hours
Refrigerated (2-8°C or 36-46°F):  Acceptable with approval of the Flow Cytometry supervisor or lab director.
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Refrigerated (2-8°C or 36-46°F): 7 days

Laboratory

Royal Oak Flow Cytometry Laboratory.

Performed

Monday - Friday, Saturday until 12:00 p.m.
Results available in 1-2 days.

Reference Range

Lymphocyte Subset Quantitation Reference Range

Age CD3(mill/L) CD4(mill/L)
0-6 months 3505-5009 2780-3908
6-12 months 3409-4575 2630-3499
12-18 months 3156-3899 2307-2864
18-24 months 2766-3508 1919-2472
24-30 months 2324-3295 1538-2213
30-36 months 1923-3141 1216-2000
3-18 years 1072-3890 562-2692
Adult 730-2460 433-1722

 

Age CD8(mill/l) CD19(mill/l) CD56(mill/l) CD4/CD8 ratio
Less than 3 years 351-2479 422-3345   1.2-6.2
3-18 years 331-1445 200-1259   0.98-3.24
Adult 143-921 44-683 42-352 0.6-6.2

Test Methodology

Immunophenotyping by Multiparameter Flow Cytometry.

Interpretation

This assay includes: absolute lymphocyte count, CD3 total T-cells, CD4 T-helper cells, CD8 T-suppressor cells, CD56 natural killer cells, CD19 B-cells, and CD4:CD8 ratio.

Clinical Utility

The Lymphocyte Subset Quantitation test may be used to assess patients with HIV infection or possible immunodeficiency.

CPT Code

86355, 86357, 86359, 86360, 88184.

Test Codes

EPIC: LAB6416, SOFT: LYSBG

Last Updated

08/29/2017

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This directory currently reflects information only for specimens collected and/or processed at the Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.