800-551-0488

Lab results are too important to go anywhere else

Beaumont Laboratory

Sjogren SSA Antibody

Sjogren, Anti-Ro, Ro Antibodies, SSA Antibodies, Antrim #30964, EPIC: LAB5767, SOFT: SJAA

Specimen Collection Criteria

Collect: One Gold-top SST tube.

Physician Office/Drawsite Specimen Preparation

Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Refrigerate (2-8°C or 36-46°F) the centrifuged collection tube within two hours of collection. (Minimum Serum: 0.5 mL)

Preparation for Courier Transport

Transport: Centrifuged collection tube, refrigerated (2-8°C or 36-46°F). (Minimum Serum: 0.5 mL)

Rejection Criteria

  • Plasma specimens.
  • Heat-inactivated specimens.
  • Severely lipemic or hemolyzed specimens.

Storage

Specimen Stability for Testing:

Centrifuged SST Tubes and Microtainers® with Separator Gels
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): Unacceptable

Red-top Tubes and Microtainers® without Separator Gels
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Serum Specimens (Pour-Overs)
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 2 months

Specimen Storage in Department Prior to Disposal:

Refrigerated (2-8°C or 36-46°F): 7 days

Laboratory

Royal Oak Special Testing Laboratory

Performed

Monday, Wednesday, Friday.
Results available within 2 business days.

Reference Range

Negative: Less than 100 AU/mL.
Equivocal: 100-120 AU/mL.
Positive: Greater than 120 AU/mL.

Test Methodology

Multiplex Bead Immunoassay.

Interpretation

SSA (Ro) antibodies are seen in 70-75% of Sjogren's, 30-40% of SLE and 5-10% of progressive systemic sclerosis (PSS).

The antibody to SSA (Ro) occurs in 62% of patients with "ANA negative" SLE, 63% of patients with subacute cutaneous erythematosus, 75% of the homozygous C2-deficient patients with an SLE-like picture, and in 80% of patients with Sjogren's syndrome who have vasculitis.

Pregnant SLE patients should be screened for anti-SSA antibodies. These antibodies have been closely associated with the development of neonatal congenital heart block and neonatal lupus.

Fluctuating titers of anti-SSA antibodies have been seen in some SLE patients. In these patients changes in anti-SSA levels often correlated with disease activity and anti-DNA antibody levels.

SLE patients that produce both SSA and SSB antibodies have a much lower incidence of nephritis than those patients producing anti-SSA antibodies alone.

Clinical Utility

The SSA (Ro) antibody assay is used to aid in the diagnosis and prognosis of certain rheumatic autoimmune diseases.

CPT Code

86235

Test Codes

Antrim #30964, EPIC: LAB5767, SOFT: SJAA

Last Updated

05/18/2016

Microtainer® and Vacutainer® are registered trademarks of Becton, Dickinson and Company.
UroVysion® is a registered trademark of Abbott Laboratories. ThinPrep® is a registered trademark of Hologic, Incorporated.

This directory currently reflects information only for specimens collected and/or processed at the Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.