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Beaumont Laboratory

Stain, Gram, Vaginal – Farmington Hills Only

Gram Stain, EPIC: LAB8041, SOFT: FHVGS

Instructions

  • Specimens should ONLY be collected at the Farmington Hills Campus and previous Botsford Hospital Outreach Clients.
  • Vaginal gram stain is a separate orderable stain and must be ordered separately. Specimens such as urethral or genital wounds/ulcers will have a routine gram stain done as part of the genital or wound culture order.

Specimen Collection Criteria

Collect: Specimen in the ESwab collection or Amies transport systems.

Physician Office/Drawsite Specimen Preparation

  • Specimen handling should be appropriate for the specimen source submitted.
  • Swab transport collection systems should be maintained at room temperature (20-26°C or 68-78.8°F) until transport.
  • Specimens should be submitted at room temperature (20-26°C or 68-78.8°F).
  • For optimal results, smears should be prepared by the Laboratory.

Preparation for Courier Transport

Transport: Specimens should be submitted at room temperature (20-26°C or 68-78.8°F).

Rejection Criteria

  • Specimens delayed in transit (greater than 24 hours).
  • Grossly contaminated specimens
  • Dessicated material.

Storage

Specimen stability and storage varies dependent on specimen type. Please refer to the individual test pages in the Lab Test Directory for information.

Laboratory

Farmington Hills Microbiology Laboratory.

Performed

Sunday – Saturday, 7:00 a.m – 12:00 a.m (midnight).
Routine results are reported within 8 hours of receipt in the Laboratory

Reference Range

Gram stain results are dependent on the morphology and Gram reaction of organisms and amount of inflammation present. The gram stain is assessed using the Nugent scoring system.

Test Methodology

Microscopic examination of a stained specimen and use of Nugent scoring of organisms observed.

Interpretation

Indicative of bacterial vaginosis based off of the gram stain using the Nugent scoring system of relative amounts of microbial morphotypes. It can provide rapid presumptive diagnosis of infectious agents and also be used to assess the quality of clinical specimens.

Clinical Utility

The Gram stain is the most basic stain in bacteriology, delineating major structural and taxonomic groups of organisms. The Nugent scoring system is the definitive laboratory method for diagnosis of bacterial vaginosis (BV) in women of childbearing years and post-menopausal women on estrogen replacement therapy.  It can also aid in the diagnosis of vaginitis.

Reference

  1. York, M.K. 2010. Gram Stain. Clinical Microbiology Procedures Manual. 3rd ed. Garcia, L.S., Editor-in-Chief (ed). ASM Press, Washington, D.C. pp.3.2.1

CPT Code

87205.

Test Codes

EPIC: LAB8041, SOFT: FHVGS

Last Updated

11/02/2017

Microtainer® and Vacutainer® are registered trademarks of Becton, Dickinson and Company.
UroVysion® is a registered trademark of Abbott Laboratories. ThinPrep® is a registered trademark of Hologic, Incorporated.

This directory currently reflects information only for specimens collected and/or processed at the Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.