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Beaumont Laboratory

Specimen Collection Manual: Blood for Mycobacterial or Acid Fast Bacillus (AFB) Isolation

Instructions

ADULTS
  • Use one Wampole® ISOLATOR 10 mL tube for each Mycobacterial or AFB Culture ordered.
  • Each tube will hold a maximum 10 mL of blood. Minimum blood volume required is no less than 7.0 mL.
PEDIATRICS
  • Use one Pediatric Wampole® ISOLATOR 1.5 mL tube for each Mycobacterial or AFB Culture ordered.
  • Each tube will hold a maximum 1.5 mL of blood. Minimum blood volume required is no less than 1.0 mL.

Specimen Collection Criteria

Collection Notes

  • Blood volume is one of the most important variables in detection of a septic episode. Submitting less than the minimum volume of blood may compromise the quality of the blood culture.
  • Hand-write on the specimen label the time and date of collection and the name of the collector. When drawing separate sets from different sites, include this information on all the labels.
  • It is recommended that 2-3 sets of blood be obtained within 24 hours and that no blood cultures be obtained after that time unless the patient's condition changes. If at all possible, blood should be obtained prior to the administration of antimicrobial agents.
  • More than 3 ordered blood cultures requires the approval of the Microbiology Medical or Technical Director.

Procedure for VENIPUNCTURE COLLECTION:

  • Decontaminate the stopper of the Wampole® ISOLATOR tube using a clean 70% isopropyl alcohol wipe. Allow alcohol to dry completely.
  • Prepare the venipuncture site as follows:
    • Thoroughly disinfect venipuncture site by applying ChloraPrep® antiseptic on the skin using a back-and-forth friction scrub for 30 seconds.
    • Allow the ChloraPrep® to dry completely (approximately 30 seconds). Do not perform venipuncture through wet Chloraprep®. NOTE: ChloraPrep® cannot be used on infants less than 2 months old. Scrub site using isopropanol/acetone sepp followed by PVP iodine sepp from center outward in a circular direction. Iodine must dry completely.
    • Do not wipe the site with guaze. Do not touch the venipuncture site with your fingers. Do not palpate the vein after skin disinfection unless a sterile glove is worn.
  • Perform the venipuncture using the Wample® ISOLATOR tube with an evacuated tube system or syringe with safety collection set (butterfly) needle. Use Vacutainer® transfer device to introduce blood into the Wampole® ISOLATOR tube.
  • It is very important to collect adequate blood volumes per Wampole® ISOLATOR tube.
  • If other tests are ordered, obtain the blood culture first. When absolutely essential, another tube may be collected before the blood culture but only when the stopper has been decontaminated with 70% isopropyl alcohol. Exceptions are Ionized Calcium and Venous pH.
  • Send Wampole® ISOLATOR tubes to the Microbiology Laboratory immediately.

Procedure for CENTRAL LINE COLLECTION

  • Avoid drawing blood through an indwelling intravenous or intra-arterial catheter unless it is needed specifically to evaluate a potential catheter-related infection or if venous access is NOT available. For proper interpretation of catheter cultures, it is recommended that venipuncture blood be obtained for a second blood culture.
  • Decontaminate the stopper of the Wampole® ISOLATOR tube using a clean 70% isopropyl alcohol wipe. Allow alcohol to dry completely.
  • Clean port with alcohol prep and allow to dry.
  • Collect blood into a syringe and fill the Wampole® ISOLATOR tubes as described above. Send to the Microbiology Laboratory immediately. 

Processing

  • Blood Cultures should be transported to the Microbiology Laboratory immediately.
  • If Wampole® ISOLATOR tubes are being sent through the pneumatic tube system, they MUST be safely cushioned between two foam inserts within the carrier.
  • Do NOT refrigerate inoculated Wampole® ISOLATOR tubes. Tubes may be held at room temperature (20-26°C or 68-78.8°F) for no longer than 4 hours prior to processing. Protect from direct sunlight. 

Last Updated

07/14/2017

Microtainer® and Vacutainer® are registered trademarks of Becton, Dickinson and Company.
UroVysion® is a registered trademark of Abbott Laboratories. ThinPrep® is a registered trademark of Hologic, Incorporated.

This directory currently reflects information only for specimens collected and/or processed at the Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.