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Beaumont Laboratory

Specimen Collection Manual: Urine for Culture

Instructions

Collection Preparation

  1. A first morning specimen is optimal.
  2. The patient must wash his/her hands.
  3. Open the collection system and remove the blue-top sterile collection-transfer container and towelettes, as seen below.
    NOTE: The blue collection cup used ONLY for collection and transfer of the urine specimen. It should NOT be used to transport the specimen to the Laboratory as it may leak when in transit.
    SAFETY WARNING: Avoid contact with the recessed needle beneath the yellow sticker on the blue-cap.
  4. Prior to genital cleansing, unscrew the cap of the urine specimen container. Place cap on the counter with the attached blue aspiration nozzle facing upward. To avoid contamination, do not touch the inside of the cup, cap, or aspiration nozzle.

Specimen Collection Criteria

Clean Catch Instructions

  1. The patient should cleanse themself with the towelettes as follows:
    • Males:
      • Wipe the head of the penis in a single motion with the first towelette. Repeat with the second towelette. (Uncircumcised patients should hold the foreskin back before cleansing.)
      • Urinate a small amount into the toilet. This flushes most contaminants from the urethra.
      • After a single stream is achieved, pass the specimen cup into the stream and obtain urine sample.
    • Females:
      • Sit comfortably on the toilet and swing one knee to the side as far as possible.
      • With the forefingers of one hand, spread the outer folds of the labia and keep them apart during the rest of the collection procedure.
      • With the other hand, wipe the inner labial folds front to back in a single motion with the first towelette.
      • Wipe down through the center of the labial folds with the second towelette.
      • Keep the labia separated and urinate a small amount into the toilet. This flushes most contaminants from the urethra.
      • After a single stream is achieved, pass the specimen cup into the stream and obtain sample.
  2. The cup should be at least 1/4 to 1/2 full.
  3. To prevent contamination of specimen with skin flora, the patient should remove the cup before the flow of urine stops and before releasing the labia or penis.
  4. The patient should then completely empty their bladder of urine into the toilet.
  5. Wipe off the outside of the container after replacing the cap.
  6. The patient must wash their hands.
  7. Label the specimen and immediately proceed to the urine transfer steps listed below.

Invasive Urine Specimen Instructions

  1. Specimen types include urine obtained through a straight catheter, cystoscopy, suprapubic bladder, tap, etc.
  2. Obtain a minimum of 10 mL of urine.
  3. Unscrew the blue cap of the sterile cup and place the cap on the counter with the attached blue aspiration nozzle facing upward. Do not touch the inside of the cup, the cap, or the straw.
  4. Place the urine in the cup and securely attach the cap to the cup.
  5. Wipe off the outside of the container after replacing the cap.
  6. Wash hands.
  7. Label the specimen and immediately proceed to the urine transfer steps listed below.

Processing

Urine Culture/Urinalysis Instructions (Between 3.0 mL and 10.0 mL of Urine)

If the urine volume within the blue-cap container is below the level of the attached blue aspiration nozzle an insufficient amount of urine will be transferred into the Culture and/or Urinalysis tube(s). In this instance, to transfer all available urine:

  1. Remove (unscrew) the blue cap and place it on the counter with the attached blue aspiration nozzle facing up.
  2. Open and remove the contents of the Culture and/or Urinalysis “straw” collection kit (shown below), making sure to avoid touching the white straw and refraining from allowing it to touch any surfaces.
  3. Place the white straw into the urine container by holding the outside of the white plastic hub.
  4. Disinfect the gray stopper of the Culture tube and/or the yellow stopper of the Urinalysis tube with an alcohol swab and allow to dry.
  5. Invert the evacuated tube(s) onto the collection needle recessed in the white plastic hub. Firmly press the tube onto the collection needle.
  6. Each tube will automatically fill with urine. When the tube is full or the required minimum of 3.0 mL is transferred, remove the tube from the collection needle. Invert the tube several times to mix.
  7. Securely replace (screw) the blue cap container.
  8. Properly label each evacuated tube (gray and/or yellow top) with a PPID barcode label if available.  If no PPID label is available, label the specimen with the patient name and date of birth.
  9. Send the tube(s) to the Laboratory.
  10. Properly discard any remaining urine. Dispose the blue-cap of the collection cup and transfer straw into an appropriate sharps biohazard container.

SAFETY WARNING: Avoid contact with the recessed needle inside the white plastic hub.

 Urine Culture/Urinalysis Transfer Instructions (Less than 3.0 mL of Urine)

  1. Remove (unscrew) the blue-cap from the urine collection container. Remove (unscrew) the orange-cap from the sterile specimen container shown below. Place both caps on the counter with the inner surface facing upward.
  2. Transfer (pour) all fluid from the urine collection container into the container that had the orange-cap.
  3. Securely (screw) replace both the blue-cap and orange-cap onto their respective containers.
  4. Properly label the orange-cap container with a PPID barcode label if available.  If no PPID label is available, label the specimen with the patient name and date of birth.
  5. Deliver the orange-cap container to the Laboratory for urine culture or urinalysis within 2 hours (room temperature) or 24 hours (refrigerated):
Test Minimum Volume Container (Shown Above)
Culture 0.5 mL Orange sterile screw-capped container, tightly capped.
Urinalysis 1.0 - 3.0 mL Orange sterile screw-capped container, tightly capped.

Last Updated

05/05/2017

Microtainer® and Vacutainer® are registered trademarks of Becton, Dickinson and Company.
UroVysion® is a registered trademark of Abbott Laboratories. ThinPrep® is a registered trademark of Hologic, Incorporated.

This directory currently reflects information only for specimens collected and/or processed at the Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.