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Beaumont Laboratory

Specimen Collection Manual: Tissue (Skin, Sub-Cutaneous, Etc.), Fluid Collection - Viral

Instructions

Materials/Supplies

  • Sterile syringe, 2-5 mL.
  • Sterile screw-capped collection container.
  • UTM or UVT transport medium.

Specimen Collection Criteria

  • Tissues: Collect tissues aseptically taking care to prevent cross-contamination when specimens are taken from multiple sites.
    • Use different tubes of transport media for tissue specimens from different sites.
    • Autopsy specimens should be collected within 24 hours of the time of death
  • Vesicle Fluid Collection: Select a vesicle containing clear fluids. The efficiency of virus detection decreases significantly when pustular fluids are collected.
    • Aseptically unroof the lesion and blot the excess fluid with a swab.
    • Gently rub the base of the lesion with a flocked ESwab to collect infected epithelial cells.
    • Place the swab in viral transport medium and break the shaft at the perforation so that it will fit into the vial.
  • Fluids: Aspirate into a sterile syringe (2-5 mL). Transfer the aspirate into a sterile, screw-capped collection container.

Rejection Criteria

  • Do not submit tissues for culture in formalin, or in containers that ever contained formalin.
  • Do not use expired media. Check expiration date on all media prior to use.    

Processing

  • Small tissue specimens should be placed into viral transport medium.
    If the Specimen is for: Then:
    Viral Culture Small tissue specimens should be placed into UTM or UVT transport medium.
    Fluids Do NOT place into UTM or UVT. Submit in a sterile, screw-capped collection container.
  • Send specimens to the Laboratory on wet ice or with a cold pack. Do not freeze. Specimens may be stored refrigerated (2-8°C or 36-46°F) for up to 24 hours. If longer delays are anticipated, specimens should be frozen at -70°C.
UTM/UVT

Last Updated

12/12/2014

Microtainer® and Vacutainer® are registered trademarks of Becton, Dickinson and Company.
UroVysion® is a registered trademark of Abbott Laboratories. ThinPrep® is a registered trademark of Hologic, Incorporated.

This directory currently reflects information only for specimens collected and/or processed at the Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.