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Vitamin B2 Level

Riboflavin, Vitamin B2

Test Codes

EPIC: LAB6179, Beaker: XVTB2, Mayo: VITB2

Department

Send Outs

Instructions

The patient must be fasting 12-14 hours prior to specimen collection (for infants, collect specimen just prior to next schedule feeding).

Specimen Collection Criteria

Collect: One Dark Green-top Lithium or Sodium Heparin tube.
Also acceptable: Light-green top (sodium or lithium heparin plasma gel).

• Protect specimen from light.
• Send specimen for processing immediately after collection.

Physician Office/Draw Specimen Preparation

Protect specimen from light. Centrifuge to separate plasma from cells within 2 hours of collection. Transfer plasma to an amber plastic transport tube and freeze (-20°C/-4°F or below).

Preparation for Courier Transport

Transport: 2.0 mL light-protected plasma, frozen (-20°C/-4°F or below). (Minimum: 0.50 mL)

Rejection Criteria

• Specimens not protected from light.
• Grossly lipemic specimens.
• Grossly icteric specimens
• Specimens not collected and processed as indicated.

In-Lab Processing

Protect specimen from light. Centrifuge to separate plasma from cells within 2 hours of collection. Transfer plasma to an amber plastic transport tube and freeze (-20°C/-4°F or below).

Transport: 2.0 mL light-protected plasma, frozen (-20°C/-4°F or below). (Minimum: 0.50 mL)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 72 hours
Refrigerated (2-8°C or 36-46°F): 28 days
Frozen (-20°C/-4°F or below): 28 days

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Mayo Medical Laboratories, Rochester, MN.

Performed

Monday, Wednesday, Friday
Results available 3-5 days.

Reference Range

By report.

Test Methodology

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).

Interpretation

By report.

Clinical Utility

The Vitamin B₂ (Riboflavin) assay aids in the evaluation of patients with ariboflavinosis and in patients who present with chronic, progressive nerve compression disorders such as carpal or tarsal tunnel syndrome.

Ariboflavinosis is the dietary deficiency of Vitamin B₂ (Riboflavin). This deficiency causes a syndrome characterized by sore throat, hyperemia, and edema of the pharyngeal and oral mucous membranes, cheilitis, angular stomatitis, glossitis, corneal vascularization, dyssebacia with dermatitis, and normochromic normocytic anemia.

Deficiencies of riboflavin and vitamin B₆ have been suggested as potentiating factors for chronic progressive nerve compression disorders, such as carpal tunnel syndrome.

CPT Codes

84252
LOINC:  2924-9

Contacts

Last Updated

10/2/2023