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Thyroid Peroxidase Autoantibody

Anti-microsomal antibody, anti-TPO, anti-Thyroid Peroxidase, Thyroid Peroxidase Antibody (TPO)

Test Codes

Antrim #30530, EPIC: LAB5821, SOFT: ATA

Department

Special Chemistry

Specimen Collection Criteria

Collect: One Gold-top SST tube.

Physician Office/Draw Specimen Preparation

Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Refrigerate (2-8°C or 36-46°F) the centrifuged collection tube within eight hours of collection. (Minimum Serum: 0.5 mL)

Preparation for Courier Transport

Transport: Centrifuged collection tube, refrigerated (2-8°C or 36-46°F). (Minimum Serum: 0.5 mL)

Rejection Criteria

Lipemic or icteric specimens.

Grossly hemolyzed specimens.

In-Lab Processing

Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Room temperature is acceptable for a maximum of eight hours. (Minimum Serum: 0.5 mL)

Storage

Specimen Stability for Testing:

Centrifuged SST Tubes and Microtainers^® with Separator Gels
Room Temperature (20-26°C or 68-78.8°F): 8 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): Unacceptable

Red-top Tubes and Microtainers^® without Separator Gels
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Serum Specimens (Pour-Overs)
Room Temperature (20-26°C or 68-78.8°F): 8 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 6 months

Specimen Storage in Department Prior to Disposal:

Refrigerated (2-8°C or 36-46°F): 7 days

Laboratory

Royal Oak Special Testing Laboratory

Performed

Monday – Friday.
Results available within 2 business days.

Reference Range

Less than or equal to 35 IU/mL.

Test Methodology

Chemiluminescence Immunoassay.

Interpretation

TPO autoantibody concentrations correlate with the degree of lymphoid infiltration of the thyroid gland. Low concentrations of TPO autoantibody predict at-risk pregnancy. TPO autoantibodies are also detected in some patients with other autoimmune disease who have normal thyroid function.

Clinical Utility

Autoantibodies to thyroid peroxidase (TPO), which is the major antigen of thyroid microsomes, can be detected in the serum of patients with autoimmune thyroid disease (e.g., Hashimoto, Graves).

CPT Codes

86376, 56477-3

Contacts

Last Updated

1/13/2023