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Parvovirus B19 by PCR

Test Codes

EPIC: LAB6315, Beaker: XPARV, ARUP: 60043

Department

Send Outs

Specimen Collection Criteria

Specimen Collection Criteria

Collect (preferred specimen): One Lavender-top EDTA tube, Gold-top SST tube, or plain Red-top tube.

Also acceptable: Amniotic fluid, Cerebrospinal Fluid (CSF), Synovial Fluid, paraffin-embedded tissue, or fresh tissue.

Specimen source is required.

Physician Office/Draw Specimen Preparation

• Centrifuge to separate plasma or serum from cells. Transfer serum or plasma to a plastic transport tube and freeze (-20°C/-4°F or below).
• Maintain fluid specimens frozen (-20°C/-4°F or below) prior to transport.
• Fresh tissue must be frozen (-20°C/-4°F or below) immediately.
• Paraffin-embedded tissue should be maintained at room temperature (20-26°C or 68-78.8°F).

Preparation for Courier Transport

Transport: 1.0 mL serum, plasma, fluid, or fresh tissue, frozen (-20°C/-4°F or below), or paraffin-embedded tissue, at room temperature (20-26°C or 68-78.8°F). (Minimum: 0.5 mL)

Rejection Criteria

• Heparinized specimens.
• Frozen whole blood.
• Bone marrow specimens.
• Specimens in non-sterile or leaking containers.
• Specimens not collected and processed as indicated.

In-Lab Processing

• Centrifuge to separate plasma or serum from cells. Transfer serum or plasma to a plastic transport tube and freeze (-20°C/-4°F or below).
• Maintain fluid specimens frozen (-20°C/-4°F or below) prior to transport.
• Fresh tissue must be frozen (-20°C/-4°F or below) immediately.
• Paraffin-embedded tissue should be maintained at room temperature (20-26°C or 68-78.8°F).

Transport: 1.0 mL serum, plasma, fluid, or fresh tissue, frozen (-20°C/-4°F or below), or paraffin-embedded tissue, at room temperature (20-26°C or 68-78.8°F). (Minimum: 0.5 mL)

Storage

Specimen Stability for Testing:

Fresh Tissue
Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): 6 months

Paraffin-Embedded Tissue
Room Temperature (20-26°C or 68-78.8°F): Indefinite
Refrigerated (2-8°C or 36-46°F): Indefinite
Frozen (-20°C/-4°F or below): Unacceptable

All Other Specimen Types
Room Temperature (20-26°C or 68-78.8°F): 24 hours
Refrigerated (2-8°C or 36-46°F): 5 days 
Frozen (-20°C/-4°F or below): 6 months

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions. 

Laboratory

Sent to ARUP Laboratories, Salt Lake City, UT.

Performed

Monday, Wednesday, Friday.
Results available in 2-5 days.

Reference Range

Negative: Parvovirus B19 DNA not detected by PCR.
Positive: Parvovirus B19 DNA detected by PCR.

Test Methodology

Qualitative Polymerase Chain Reaction (PCR).

Interpretation

• A negative result does not rule out the presence of PCR inhibitors in the patient specimen or Parvovirus DNA in concentrations below the level of detection of the assay.
• This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.

CPT Codes

87798

Contacts

Last Updated

11/3/2023