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Iso (Allo) Hemagglutinin Titer

Test Codes

EPIC: LAB5291, Beaker: ISOTI

Department

Blood Bank

Specimen Collection Criteria

Collect: One Pink-top EDTA tube, properly labeled. (Minimum Volume: 5.0 mL)

It is extremely important that blood samples are accurately labeled. Inpatient labels must include the following information:

1. Patient's Name: Complete name. If the patient uses a first initial followed by a middle name, both must appear in proper order on the label.
2. Wrist Band Number: Include the prefix letters, 4 numbers, and a suffix letter (example: BR1234T). Specimens lacking this complete information cannot be used for blood transfusion purposes and require recollection.
3. Hospital Identification Number: The seven digit medical record number must be included on the label.
4. Date and Time Collected
5. Identification of Collector: Employee ID number or first initial and complete last name must be on the sample tube or documented in the computer system.

For Beaumont Reference Laboratory (Outreach) patients, please refer to the Laboratory Bulletin on Laboratory Specimens: How to Label Correctly. 

Physician Office/Draw Specimen Preparation

Maintain specimen at room temperature (20-26°C or 68-78.8°F) or refrigerated (2-8°C or 36-46°F) prior to transport.

Preparation for Courier Transport

Transport: Whole blood at room temperature (20-25°C or 68-77°F) or refrigerated (2-8°C or 36-46°F).

Rejection Criteria

• Improperly labeled specimens.
• Specimens with inadequate volume.
• Specimens collected in inappropriate collection  tubes.
• Specimens contaminated with IV fluids.
• Grossly hemolyzed specimens will be tested only in emergency situations. 
  

In-Lab Processing

Centrifuge the specimen upon receipt in the Laboratory.

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 24 hours
Refrigerated (2-8°C or 36-46°F): 72 hours
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Refrigerated (2-8°C or 36-46°F): Specimens are held for a minimum of 7 days.

*Once a sample arrives in Blood Bank, it cannot be shared with other laboratories.

Laboratory

Royal Oak Transfusion Medicine Laboratory (Blood Bank)

Performed

Monday – Friday, 6:30 am - 3:00 pm.
Results available within 24 hours.

Test Methodology

Hemagglutination-tube.

Interpretation

By report.

Clinical Utility

• Ordinarily, people possess antibodies directed toward the A and/or B antigen absent from their own rbcs. Titration of these antibodies is most often requested for the assessment of solid organ transplant compatibility.
• Historically, Iso Hemagglutinin Titers were obtained to check for immunodeficiency in young children (9 months to 2 years) suspected to have a disease state associated with absent or abnormally low allohemagglutinins.

Reference

1. AABB Technical Manual, current edition.
2. Royal Oak Blood Bank Procedure Manual.

CPT Codes

86940

Contacts

Last Updated

10/29/2023