Fungitell
Beta-D-Glucan
Test Codes
MiraVista #317, CSF: EPIC: LAB6983, Beaker: XFGTC, Serum: EPIC: LAB6982, Beaker: XFGTS
Department
Send Outs
Specimen Collection Criteria
Collect one of the following specimen types:
- CSF: 0.5 mL in a sterile collection container. (Minimum: 0.2 mL)
- Blood: One Gold-top SST tube or one plain Red-top tube. (Minimum: 0.2 mL)
Send specimen for processing immediately after collection.
Physician Office/Draw Specimen Preparation
CSF: Maintain specimen refrigerated (2-8°C or 36-46°F).
Blood: Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).
Preparation for Courier Transport
Transport: CSF in a sterile collection container and/or serum, refrigerated (2-8°C or 36-46°F). (Minimum CSF/Serum: 0.2 mL)
Rejection Criteria
- Hemolyzed specimens.
- Icteric specimens.
- Lipemic specimens.
- Specimens not collected and processed as indicated.
In-Lab Processing
CSF: Maintain specimen refrigerated (2-8°C or 36-46°F).
Blood: Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).
Transport: CSF in a sterile collection container and/or serum, refrigerated (2-8°C or 36-46°F). (Minimum CSF/Serum: 0.2 mL)
Storage
Specimen Stability for Testing:
Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): 5 days
Frozen (-20°C/-4°F or below): Indefinite
Specimen Storage in Department Prior to Disposal:
Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.
Laboratory
Sent to MiraVista Diagnostics, Indianapolis, IN.
Performed
Monday – Friday.
Results available in 2-4 days.
Reference Range
Negative: Less than 60 pg/mL.
Indeterminate: 60-79 pg/mL.
Positive: 80 - greater than 500 pg/mL.
Test Methodology
Colorimetric assay utilizing a (1→3)-β-D-Glucan-specific Limulus Amebocyte Lysate (LAL) reagent.
Interpretation
The Fungitell assay does not detect certain fungal species such as genus Cryptococcus which produces very low levels of (1→3)-β-D-Glucan. The assay also does not detect Zygomycetes such as Absidia, Mucor, and Rhizopus, which are not known to produce (1→3)-β-D-Glucan. In addition, the yeast phase of Blastomyces dermatitidis produces little (1→3)-β-D-Glucan and may not be detected by the assay.
Clinical Utility
This assay aids in the presumptive diagnosis of invasive fungal disease in at-risk patients.
CPT Codes
87449
LOINC: 62468-4
Contacts
Send Outs Laboratory – RO
248-551-9045
Name: Send Outs Laboratory – RO
Location:
Phone: 248-551-9045
Last Updated
3/4/2022
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