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Fibrin Degradation Products

FSP, FDP, Fibrin Breakdown products, Fibrin Split Products

Test Codes

Temporary code: XMISC LAB6433
Ask order question: free text “FDP”

Department

Coagulation

Instructions

Specimens with hematocrit values greater than 55% must have anticoagulant adjusted. Specimens must be re-collected using a specified whole blood to sodium citrate ratio. Contact the performing Laboratory for specific instructions.

Specimen Collection Criteria

Collect: One Light Blue-top 3.2% Sodium Citrate tube OR One Pediatric Light Blue-top Sodium Citrate tube (1.8 mL)

TUBE MUST BE FULL. 

Physician Office/Draw Specimen Preparation

For optimal results, maintain whole blood specimen at room temperature (20-26°C or 68-78.8°F) prior to transport. Transport whole blood to the Laboratory within 1 hour of collection. If transport within 1 hour is not possible, the following procedure must be followed for accurate results:

1. Centrifuge the capped tubes at 1500 x g for 15 minutes.
2. Transfer plasma with plastic pipette into a plastic polypropylene centrifuge tube, cap and centrifuge an additional 15 minutes at 1500 x g to obtain platelet poor plasma (PPP) which has a platelet count less than 10 bil/L. Plasma with a platelet count of less than 10 bil/L is critical for accurate results.
3. Immediately remove only the top two-thirds of the PPP and transfer into a screw cap, 5 ml, 75 x 13 mm, round base, polypropylene tube.  
4. Freeze (-20°C/-4°F or below) the PPP immediately.
5. Transport frozen on DRY ICE. Specimen must remain frozen during transport. 

Preparation for Courier Transport

Transport: Whole blood at room temperature (20-25°C or 68-77°F), or plasma obtained through the process listed above, frozen (-20°C/-4°F or below). 

Rejection Criteria

• Glass tubes and 3.8% Sodium Citrate tubes.
• Specimens that are clotted or grossly hemolyzed.
• Inappropriate specimen volume.
• Thawed or partially thawed specimens.

In-Lab Processing

Whole blood specimens must be processed as follows upon receipt in the Laboratory: 

1. Centrifuge the capped tubes at 1500 x g for 15 minutes.
2. Transfer plasma with plastic pipette into a plastic polypropylene centrifuge tube, cap and centrifuge an additional 15 minutes at 1500 x g to obtain platelet poor plasma (PPP) which has a platelet count less than 10 bil/L. Plasma with a platelet count of less than 10 bil/L is critical for accurate results.
3. Immediately remove only the top two-thirds of the PPP and transfer into a plastic tube.
4. Test or freeze specimen according to lab protocol.

Storage

Specimen Stability for Testing:

Whole Blood
Room Temperature (20-26°C or 68-78.8°F): 1 hour
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Platelet Poor Plasma (PPP)
Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): 30 days

Specimen Storage in Department Prior to Disposal:

Frozen (-20°C/-4°F or below): 30 days

Laboratory

Royal Oak Send Outs Laboratory
Corewell Health Reference Laboratory, Grand Rapids, MI 

Performed

Sunday – Saturday.
Results available within 24-48 hours.

Reference Range

Normal Value: Less than 5 mcg/mL.

Test Methodology

Rapid latex slide agglutination test using mouse anti-FDP antibodies.

Interpretation

Free D and E domains as well as X and Y fragments detected by this method may be produced by degradation of either fibrin clot or circulating fibrinogen.

In cases where the FDP assay is positive and it is desired to distinguish lysis of fibrin from intravascular fibrinolysis, it is recommended to suggest the D-Dimer assay.

Clinical Utility

Useful for the detection and semi-quantitation of fibrin/fibrinogen degradation products in plasma with latex particles which are coated with monoclonal antibodies to FDP. 

Abnormal fibrinolytic and or fibrinogenolytic activity demonstrated by elevated levels of FDP in plasma may be seen in the following:

• DIC
• Eclampsia
• Carcinoma
• Post-operative
• Cardiac or hepatic disorders
• Fibrinolysis
• Pulmonary embolism
• Deep vein thrombosis

Limitations:

• The slightest coagulation of the specimen may produce false positive reactive with the FDP plasma.
• High levels of rheumatoid factor (RF) may lead to false agglutinations. Any suspected presence of RF should be confirmed by a separate test for RF.
• The FDP Plasma test is insensitive to heparins up to 2 IU/mL.
• The presence of anti-mouse of antibodies in certain subjects may lead to aberrant test results.

CPT Codes

85362

Contacts

Last Updated

3/11/2024