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CEBPA Mutation

Test Codes

EPIC: LAB7099, Beaker: XCEBG, U of M: CEBPA

Department

Send Outs

Specimen Collection Criteria

Collect (preferred specimen): Blood in one Lavender-top EDTA or Yellow-top ACD tube. (Minimum: 1.0 mL)
Also acceptable: Bone marrow aspirate in one Lavender-top EDTA or Yellow-top ACD tube (Minimum: 0.5 mL) or Extracted DNA (50 ng/mcL).

Invert several times to mix blood or bone marrow.

Physician Office/Draw Specimen Preparation

Do not centrifuge. Maintain specimen in original collection tube, refrigerated (2-8°C or 36-46°F) prior to transport.

Preparation for Courier Transport

Transport: 5.0 mL blood or 3.0 mL bone marrow, refrigerated (2-8°C or 36-46°F) in original collection tube. (Minimum: 1.0 mL blood or 0.5 mL bone marrow)

Rejection Criteria

• Frozen specimens.
• Centrifuged specimens.
• Specimens in clot or SST tubes.
• Formalin-fixed, paraffin-embedded specimens
• Specimens not collected and processed as indicated.

In-Lab Processing

Do not centrifuge. Maintain specimen in original collection tube, refrigerated (2-8°C or 36-46°F) prior to transport.

Transport: 5.0 mL blood or 3.0 mL bone marrow, refrigerated (2-8°C or 36-46°F) in original collection tube. (Minimum: 1.0 mL blood or 0.5 mL bone marrow)

Storage

Specimen Stability for Testing:

Room temperature (20-26°C or 68-78.8°F): 48 hours
Refrigerated (2-8°C or 36-46°F): 48 hours
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to the University of Michigan, Ann Arbor, MI.

Performed

Monday – Thursday.
Results available in 3-10 days.

Reference Range

Negative (no mutations).

Test Methodology

Multiplex Polymerase Chain Reaction (PCR) followed by Sequencing Analysis.

CPT Codes

81218

Contacts

Last Updated

8/16/2023