Lab Test

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Acute Hepatitis Panel

Test Codes

EPIC: LAB5216, AHPT

Department

Chemistry

Instructions

Acute Hepatitis Panel includes:

Specimen Collection Criteria

Collect (preferred specimen): One Gold-top SST tube. (Minimum Whole Blood: 4.0 mL)
Also acceptable: One Lavender-top EDTA tube. (Minimum Whole Blood: 3.0 mL) 

Physician Office/Draw Specimen Preparation

  • Let SST specimens clot 30-60 minutes then immediately centrifuge to separate serum from cells. Refrigerate (2–8°C or 36–46°F) the centrifuged SST tube within two hours of collection. (Minimum: 2.0 mL)
  • Centrifuge Lavender-top tube immediately to separate plasma from cells. Transfer plasma to a plastic transport tube and refrigerate (2–8°C or 36–46°F) within twelve hours of collection. (Minimum: 2.0 mL)

Preparation for Courier Transport

Transport: Centrifuged SST tube or plasma aliquot, refrigerated (2-8°C or 36-46°F). (Minimum: 2.0 mL serum or plasma)

Rejection Criteria

  • Hemolyzed specimens.
  • Severely lipemic specimens.
  • Specimens that have gross bacterial contamination.
  • Red-top tubes with serum not separated from cells within two hours of collection.
  • Lavender-top tubes with plasma not separated from cells within twelve hours of collection.

In-Lab Processing

Let SST specimens clot 30–60 minutes. Centrifuge SST, Lavender-top tubes, or Microtainers® to separate serum or plasma from cells. Deliver immediately to the appropriate testing station.

Storage

Specimen Stability for Testing:

Centrifuged SST Tubes and Microtainers® with Separator Gel
Room Temperature (20–26°C or 68–78.8°F): 2–4 hours
Refrigerated (2–8°C or 36–46°F): 7 days
Frozen (-20°C/-4°F or below): Unacceptable

Red-top Tubes and Microtainers® without Separator Gel
Room Temperature (20–25°C or 68–77°F): 2–4 hours
Refrigerated (2–8°C or 36–46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Serum Specimens (Pour-Overs)
Room Temperature (20–26°C or 68–78.8°F): 2–4 hours
Refrigerated (2–8°C or 36–46°F): 7 days
Frozen (-20°C/-4°F or below): 7 days

Plasma Specimens (Aliquots)
Room Temperature (20–26°C or 68–78.8°F): 12 hours
Refrigerated (2–8°C or 36–46°F): 7 days
Frozen (-20°C/-4°F or below): 7 days

Specimen Storage in Department Prior to Disposal:

Refrigerated (2–8°C or 36–46°F): 7 days

Laboratory

Dearborn Chemistry Laboratory
Royal Oak Automated Chemistry Laboratory 

Performed

Sunday – Saturday, 24 hours a day.
Results available within 24 hours of receipt in the Laboratory.

Reference Range

Hepatitis A Antibody (IgM):  Negative.
Hepatitis B Core Antibody (IgM):  Negative.
Hepatitis B Surface Antigen:  Negative.
Hepatitis C Antibody:  Negative.

Test Methodology

Chemiluminescent Immunoassay.

Clinical Utility

This panel is used in the differential diagnosis of hepatitis infections. See individual entries for more information:

Disease Reporting

This is a reportable infection and positive results will be reported to the Oakland County Health Department. For more information on reportable diseases, contact the epidemiology at 248-551-4040.

CPT Codes

80074

Contacts

Last Updated

8/24/2023

Microtainer® and Vacutainer® are registered trademarks of Becton, Dickinson and Company.
UroVysion® is a registered trademark of Abbott Laboratories. ThinPrep® is a registered trademark of Hologic, Incorporated.